The Resource Genomic imprinting : methods and protocols, edited by Andrew Ward, (eBook)

Genomic imprinting : methods and protocols, edited by Andrew Ward, (eBook)

Label
Genomic imprinting : methods and protocols
Title
Genomic imprinting
Title remainder
methods and protocols
Statement of responsibility
edited by Andrew Ward
Contributor
Subject
Genre
Language
eng
Member of
Additional physical form
Also available in print.
Cataloging source
N$T
Characteristic
unknown
Dewey number
572.8/6/028
http://library.link/vocab/relatedWorkOrContributorDate
1964-
http://library.link/vocab/relatedWorkOrContributorName
Ward, Andrew
Series statement
  • eBooks from EBSCOhost
  • Methods in molecular biology
Series volume
v. 181
http://library.link/vocab/subjectName
  • Genomic imprinting
  • SCIENCE
  • Electronic books
  • Genomic Imprinting
Label
Genomic imprinting : methods and protocols, edited by Andrew Ward, (eBook)
Link
https://atxlibrary.idm.oclc.org/login?url=http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=67696
Instantiates
Publication
Copyright
Note
  • Mode of access: World Wide Web
  • Access restricted to subscribing institutions
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Generation of monoparental embryos for investigation into genomic imprinting -- Deriving and propagating mouse embryonic stem cell lines for studying genomic imprinting -- Balanced translocation for the analysis of imprinted regions of the mouse genome -- Production of YAC transgenic mice by pronuclear injection -- A transgenic approach to studying imprinted genes: modified BACs and PACs -- Methylation-sensitive genome scanning -- Subtraction-hybridization method for the identification of imprinted genes -- Identification of imprinted loci by methylation: use of methylation-sensitive representational difference analys (Me-RDA) -- Ribonuclease protection -- Quantitative RT-PCR-based analysis of allele-specific gene expression -- Allele-specific In Situ hybridization (ASISH) -- RNA-FISH to analyze allele-specific expression -- Flow cytometry and FISH to investigate allele-specific replication timing and homologous association of imprinted chromosomes -- Southern analysis using methyl-sensitive restriction enzymes -- A PCR-based method for studying DNA methylation -- Bisulfite-based methylation analysis of imprinted genes -- Direct analysis of chromosome methylation --In vitro methylation of predetermined regions in recombinant DNA constructs-- In vitro
  • methylation of specific regions in recombinant DNA constructs by excision and religation -- Detection of methyl-sensitive DNA-binding proteins with possible involvement in the imprinting phenomenon -- Probing chromatin, structure with nuclease sensitivity assays -- Examining histone acetylation at specific genomic regions -- Purification of the MeCP2/histone deacetylase complex from Xenopus laevis -- Reconstitution of chromatin in vitro -- Genomic imprinting in plants
Control code
943235
Dimensions
unknown
Extent
1 online resource (xii, 377 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
  • (Sirsi) tsl-ebsco24037
  • (OCoLC)50175107
Label
Genomic imprinting : methods and protocols, edited by Andrew Ward, (eBook)
Link
https://atxlibrary.idm.oclc.org/login?url=http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&db=nlabk&AN=67696
Publication
Copyright
Note
  • Mode of access: World Wide Web
  • Access restricted to subscribing institutions
Bibliography note
Includes bibliographical references and index
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Color
multicolored
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • Generation of monoparental embryos for investigation into genomic imprinting -- Deriving and propagating mouse embryonic stem cell lines for studying genomic imprinting -- Balanced translocation for the analysis of imprinted regions of the mouse genome -- Production of YAC transgenic mice by pronuclear injection -- A transgenic approach to studying imprinted genes: modified BACs and PACs -- Methylation-sensitive genome scanning -- Subtraction-hybridization method for the identification of imprinted genes -- Identification of imprinted loci by methylation: use of methylation-sensitive representational difference analys (Me-RDA) -- Ribonuclease protection -- Quantitative RT-PCR-based analysis of allele-specific gene expression -- Allele-specific In Situ hybridization (ASISH) -- RNA-FISH to analyze allele-specific expression -- Flow cytometry and FISH to investigate allele-specific replication timing and homologous association of imprinted chromosomes -- Southern analysis using methyl-sensitive restriction enzymes -- A PCR-based method for studying DNA methylation -- Bisulfite-based methylation analysis of imprinted genes -- Direct analysis of chromosome methylation --In vitro methylation of predetermined regions in recombinant DNA constructs-- In vitro
  • methylation of specific regions in recombinant DNA constructs by excision and religation -- Detection of methyl-sensitive DNA-binding proteins with possible involvement in the imprinting phenomenon -- Probing chromatin, structure with nuclease sensitivity assays -- Examining histone acetylation at specific genomic regions -- Purification of the MeCP2/histone deacetylase complex from Xenopus laevis -- Reconstitution of chromatin in vitro -- Genomic imprinting in plants
Control code
943235
Dimensions
unknown
Extent
1 online resource (xii, 377 pages)
Form of item
online
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other physical details
illustrations.
Specific material designation
remote
System control number
  • (Sirsi) tsl-ebsco24037
  • (OCoLC)50175107

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